Reply With Quoteyep, just continue to replenish the refrigerated bottle.
thats how i do it..
I did my first split last friday. As I continue to split can I put the phyto into the same container in the fridge for does each split need a separate container?
yep, just continue to replenish the refrigerated bottle.
thats how i do it..
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Keep your fingers crossed Rob. Hopefully I will have better luck than others starting with DT"S
My inputs...
I USED to just keep topping off the bottle in the fridge until all of a sudden it went sour. I did a little experiment. For one month, I continued to top off a single bottle and at the same time, create new bottled phyto from clean containers. The clean bottled cultures, if agitated (Shaken) from time to time, but kept sealed, lasted longer than the bottle I was topping off.
I would have to assume that there is SOME die off in the older bottle and by adding new phyto to it, I just contaminated the new stuff I topped off with.
In any case, I now keep new bottles handy for storage and use each until it is gone. At that point, I sterilize them for use later. I don't continue to use the same bottle at all.
Dave
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"Fins to the left...Fins to the Right, I'm going home to play with my reef tonight..."
If you're looking for me, and I'm MIA from the board, email me at JustDavidP (at) gmail (dot) com.
Experience in aquaria, 37 years. Experience in marine, 22+ years. Experience in Reef Keeping, 8 years. Always a newbie!
yes, and i shoudl clarify on how i do this.
i have one bottle that i use, adn a second that is stored also.
starting with a full 2 liter, i use that.
as cultures continue to grow, i add them to the second 2 liter.
then when the first is gone, i trasnfer from the second bottle to the first, adn use that.
continue fillign the second bottle.
so here is how my phtyo flow works
starts in culture container.
then to bottle 2
then to bottle 1
this way i dont really have anything older than what i used to fill the second bottle.
that its all new again..
hope that makes sense.
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I have another question... I just started culturing nanno and tetra. The nanno, I combine with 35 ppm salt water. The tetra, I use 50 ppm salt water.
Can I premix a couple of gallons each and keep them sealed and stored and just use a little from the jugs when I do my splits instead of mixing a fresh back of each salinity every time I split?
well first off, i hope you mean 35 ppt not ppm (parts per trillion)
second, i am not familiar with tetra, but i have found that nano does best at a slightly lower salinity.. i keep mine at a SG of 1.019-1.020
and on the other comment, that's exactly what i do, and showed in the video. i have 4 gallons premixed with the "food" in it and ready to go as i need it
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Oops... that's what I meant, ppt. At work, I'm always thinking in ppm.
Good idea about the food in it.
yep.. if you watch the video, you will see exactly what and how i do mineOriginally Posted by mdavis203
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nice vidio
Just made my second split of my phyto culture. This batch was much darker green but there was a small amout of brown residue in the bottom of the container - what was it?
the residue is normal, over time you will see this continue
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my question is a little different.. where can i order the hard tubing?
it should be available at your LFS. almost all of them sell it.
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Rob I have made 4 splits so far of my phyto started with DT's. Do I hold the record?
how dark are they getting?
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beats my record
><((((º>`·.¸¸.·´¯`·.¸.·´¯`·...¸><((((º>
·´¯`·.¸. , . .·´¯`·.. >((((º>
"Fins to the left...Fins to the Right, I'm going home to play with my reef tonight..."
If you're looking for me, and I'm MIA from the board, email me at JustDavidP (at) gmail (dot) com.
Experience in aquaria, 37 years. Experience in marine, 22+ years. Experience in Reef Keeping, 8 years. Always a newbie!
beats the people i know that have tried it...
would love to see an image of how dark it is..
with the lighting behind it and on...
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I compared side by side the split with the new batch after I refilled with RO water and it is quite darker. I have a digital camera on order and it should be here at the end of the week. I will post a picture of the comparison of the next split - #5. I will also finally be able to post some tank pictures!!
awesome..
yes, i would love to see how its doing..
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I posted a picture of the 5th split of my phyto started with DT's in the photo gallery.
Last edited by Reefbaby; 05-21-2006 at 06:32 AM.
Congratulations bband!! Can't wait to hear what the others say! How is your tank liking the doses?
Christi
awesome.. it does look like its doing good...
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I just ordered my starter culture of Nannochloropsis oculata from www.seahorsesource.com, I will keep you all posted on how it goes.
Rob, where did you get the strainer you used in the video???
awesome, glad to see you went to them.
did you use the talkingreef coupon code to see if there is a discount?
the strainer i have is from here
the one i have is the 4" sieve with the collector
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