Splitting phytoplankton

**pdfb55** · 06-23-2006, 04:56 PM

I started a culture of phytoplankton from algae disks on last wednesday so it has been about 10 days. the culture started getting darker and darker but its been 10 days and it isn't as dark as it should be to split. I can still sort of see the light through the phytoplankton. What should i do, split it now or wait a few days?
Thanks
Kyle

**pham411** · 06-23-2006, 05:50 PM

yes split it before it crashes from lack of nutrients. there is no harm if you split it early but your cultures can crash from leaving it too long. though i heard people leaving it for a really long time.

**pdfb55** · 06-23-2006, 05:53 PM

Ok ill split it then. It should get darker faster the next time since its the second time, right?

***Rob*** · 06-23-2006, 06:45 PM

yes, it will culture faster next time...

V · 06-23-2006, 07:31 PM

give these blokes a nudge & see if u can get a home model, take the guess work out of culturing....lol

http://www.nasatech.com/Briefs/Nov00/SSC00110.html

V · 06-23-2006, 07:42 PM

this didn't load correctlt first time, so take two!

check out what the suckers look like under the scope

V · 06-23-2006, 08:10 PM

some visual aspects of culturing! ive seen some posted around here somewhere, but its easyer just posting my own source.
it takes me back to high school chemistry sitting next to the hot chicks.

arrrr the good old days!

**fat walrus** · 06-23-2006, 08:10 PM

neat picture. what type is it?

V · 06-23-2006, 08:14 PM

u know what blub, i have know idea! forgot to save the genus

**fat walrus** · 06-23-2006, 08:22 PM

Originally Posted by veriann

u know what blub, i have know idea! forgot to save the genus

short memories is a result of hanging out in chemistry class too long trying to meet those mythical hot chicks. i hung out in home economics where there were plenty of cooking and and 99% girl enrollment. i had a monopoly while you sniffed chemicals and have hallucinations surrounded by nerds. lol

V · 06-23-2006, 08:29 PM

priceless...lol

**CarmieJo** · 06-24-2006, 12:02 AM

I hung out in chemistry class with the hot boys!

**fat walrus** · 06-24-2006, 12:10 AM

Originally Posted by CarmieJo

I hung out in chemistry class with the hot boys!

carmie, i fear that you have also sniffed too many chemcals and may have hallucinated! lol.

V · 06-24-2006, 12:13 AM

nothing wrong with hot nerds, u want to come back to my place to see my helios sphere, it produces a "big bang, and all u will see is stars babe!"

**fat walrus** · 06-24-2006, 12:17 AM

Originally Posted by veriann

nothing wrong with hot nerds, u want to come back to my place to see my helios sphere, it produces a "big bang, and all u will see is stars babe!"

so what is an aussie nerd? steve with plastic pocket pen holder, or Dundee with pants 4 inches too short?

V · 06-24-2006, 12:22 AM

indeed thats pretty close blubber, about sums it up!
thats not a joke, this is a joke!

**fat walrus** · 06-24-2006, 12:26 AM

Originally Posted by veriann

indeed thats pretty close blubber, about sums it up!
thats not a joke, this is a joke!

so i guess all nerds internationally share a common pants length among other things.

V · 06-24-2006, 12:03 PM

nerds= never ever rode donkeys

***Rob*** · 06-24-2006, 03:17 PM

personally i prefer "geek"

but before we go WAY off topic...
Kyle,
did you get the help and answers you needed?
was there anything else you are looking for?

**dpmamay** · 06-24-2006, 06:25 PM

Geeks are social, nerds are loners!!!!

**CarmieJo** · 06-24-2006, 07:15 PM

My son says I am a nerd & a geek.

V · 06-24-2006, 07:25 PM

Originally Posted by pdfb55

I started a culture of phytoplankton from algae disks on last wednesday so it has been about 10 days. the culture started getting darker and darker but its been 10 days and it isn't as dark as it should be to split. I can still sort of see the light through the phytoplankton. What should i do, split it now or wait a few days?
Thanks
Kyle

ahem, yes, getting back on topic lol