actually here..found instructions for rotifers
Place Resting Rotifer cysts in a shallow wide dish that provides a large surface area to allow for gas exchange and fill with a half an inch of culture water. (Culture water for rotifers is prepared by sterilizing 250mL of
ro/di water in a micro-wave for 7-10 minutes on high. Allow the water to cool to room temperature, now using your brand of salt mix to a
specific gravity of 1.0069-1.0107.) Drip a small amount of prepared culture water into the vial of Resting Rotifers to help hydrate the cysts, shake well and pour into your shallow wide dish. Cover the dish with clear plastic (such as Saran Wrap) and provide continuous light using a flourescent 6500K light and one
actinic. (This lighting should provide you between 500-2000 lux.) Keep temperature at 77 F (25C).
Hatching will start in 1 to 3 days. At the 24 hour mark assume that hatching has begun, whether you see any rotifers or not, add a small amount of
phytoplankton (enough to turn the water a very light green colour), or add a drop or two of rotifer enrichment (just enough to slightly cloud the water.
At 48 hours transfer your culture of rotifers to a larger vessel (capable of holding 500mL). Add 500mL of prepared culture water (as outlined previously). Again feed your culture fresh
phytoplankton (just enough to turn the water a light green) or add a few drops of rotifer enrichment (just enough to turn the water cloudy).
At 72 hours (third day) simply stir and feed your live rotifer culture again.
At 96 hours (fourth day), transfer your culture to a 2 liter container and add 2 liters of prepared culture water. Again feed with
phytoplankton (add just enough
phytoplankton to turn the water a light green colour), if Roti Rich is used add just enough to cloud the water slightly. Important: In the beginning stages of culturing rotifers it is important to provide food routinely but never in excess. Excess food will impede reproduction by shifting
pH values. Secondly, always start your cultures in small volumes and slowly work to larger vessels, the reason for this is that you want your culture to always be greater than bacteria. It's nature's old rule: The strongest survive. By starting with small dense cultures and slowly working to larger vessels, will ensure success.
Continue to provide 12 to 24 hours of medium to low lighting, and maintain your culture at a temperature between 70-80 F (21-27C). Provide enough aeration to maintain good circulation.
After 7 to 10 days, your culture of rotifers should be dense enough for you to start harvesting. Rotifers are best collected by using a 53 micron plankton collector.
to TR.
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