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Old 04-25-2006, 08:34 PM   #76 (permalink)
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I did my first split last friday. As I continue to split can I put the phyto into the same container in the fridge for does each split need a separate container?
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Old 04-25-2006, 09:46 PM   #77 (permalink)
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yep, just continue to replenish the refrigerated bottle.
thats how i do it..
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Old 04-25-2006, 10:18 PM   #78 (permalink)
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Keep your fingers crossed Rob. Hopefully I will have better luck than others starting with DT"S
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Old 04-26-2006, 09:30 AM   #79 (permalink)
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My inputs...

I USED to just keep topping off the bottle in the fridge until all of a sudden it went sour. I did a little experiment. For one month, I continued to top off a single bottle and at the same time, create new bottled phyto from clean containers. The clean bottled cultures, if agitated (Shaken) from time to time, but kept sealed, lasted longer than the bottle I was topping off.

I would have to assume that there is SOME die off in the older bottle and by adding new phyto to it, I just contaminated the new stuff I topped off with.

In any case, I now keep new bottles handy for storage and use each until it is gone. At that point, I sterilize them for use later. I don't continue to use the same bottle at all.

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Old 04-26-2006, 08:12 PM   #80 (permalink)
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yes, and i shoudl clarify on how i do this.
i have one bottle that i use, adn a second that is stored also.
starting with a full 2 liter, i use that.
as cultures continue to grow, i add them to the second 2 liter.
then when the first is gone, i trasnfer from the second bottle to the first, adn use that.
continue fillign the second bottle.

so here is how my phtyo flow works
starts in culture container.
then to bottle 2
then to bottle 1

this way i dont really have anything older than what i used to fill the second bottle.
that its all new again..

hope that makes sense.
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Old 04-27-2006, 11:06 AM   #81 (permalink)
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I have another question... I just started culturing nanno and tetra. The nanno, I combine with 35 ppm salt water. The tetra, I use 50 ppm salt water.

Can I premix a couple of gallons each and keep them sealed and stored and just use a little from the jugs when I do my splits instead of mixing a fresh back of each salinity every time I split?
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Old 04-27-2006, 11:17 AM   #82 (permalink)
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well first off, i hope you mean 35 ppt not ppm (parts per trillion)

second, i am not familiar with tetra, but i have found that nano does best at a slightly lower salinity.. i keep mine at a SG of 1.019-1.020

and on the other comment, that's exactly what i do, and showed in the video. i have 4 gallons premixed with the "food" in it and ready to go as i need it
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Old 04-27-2006, 03:22 PM   #83 (permalink)
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Oops... that's what I meant, ppt. At work, I'm always thinking in ppm.

Good idea about the food in it.
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Old 04-27-2006, 03:56 PM   #84 (permalink)
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Quote:
Originally Posted by mdavis203
Oops... that's what I meant, ppt. At work, I'm always thinking in ppm.

Good idea about the food in it.
yep.. if you watch the video, you will see exactly what and how i do mine
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Old 04-27-2006, 06:06 PM   #85 (permalink)
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nice vidio
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Old 04-28-2006, 11:54 PM   #86 (permalink)
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Just made my second split of my phyto culture. This batch was much darker green but there was a small amout of brown residue in the bottom of the container - what was it?
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Old 04-29-2006, 12:27 AM   #87 (permalink)
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the residue is normal, over time you will see this continue
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Old 04-30-2006, 04:39 PM   #88 (permalink)
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my question is a little different.. where can i order the hard tubing?
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Old 04-30-2006, 07:11 PM   #89 (permalink)
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it should be available at your LFS. almost all of them sell it.
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Old 05-16-2006, 12:03 PM   #90 (permalink)
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Rob I have made 4 splits so far of my phyto started with DT's. Do I hold the record?
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Old 05-16-2006, 12:33 PM   #91 (permalink)
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how dark are they getting?
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