Nitrate Test
Medium range procedure: 2 - 100 mg/L (ppm)
(1) Fill test tube with 1 ml of water.
(2) Add 1 level scoop
NO3-1.
(3) Add 1 level scoop
NO3-3 (NO3-2 is omitted in this procedure) and swirl gently for 30 seconds.
(4) Let it stand for 3 minutes.
(5) Place the test tube on a white part of the color chart and compare colors looking from the top. If the color corresponds to a value significantly lower than 10 mg/L try using the low range procedure to obtain a better-resolved value.
Low range procedure: 0.2 - 10 mg/L (ppm)
(1) Fill test tube with 10 ml of water.
(2) Add 1 level scoop
NO3-1
(3) Add 1 drop of
NO3-2 reagent.
(4) Add level scoop of
NO3-3 and swirl gently for 30 seconds.
(5) Let it stand for 3 minutes.
(6) Place the test tube on a white part of the color chart and compare colors looking from the top. Divide the values on the chart by 10!
Note: A reading, after division, of 10 mg/L (ppm) could imply that the value is higher. Use the medium range procedure in such cases. Values on the chart are in mg/L or
ppm NO3. Clean test tube and scoop after use. Store this kit in a dry place. Keep-out of reach of Children!
Nitrite
Instructions:
1. Add with the syringe 1ml of water in the test vial.
2. Add to 1 level spoon of the
NO2 powder. Swirl the contents of the test tube gently for 20 seconds. Allow to stand for 3 minutes.
3. Place the open test vial on top of the color chart on a part of the chart which is whiteand compare the colors looking from the top. Read the corresponding
nitrite content. An intermediate color corresponds to an intermediate
nitrite content.
The
nitrite values are in
ppm nitrite. If you prefer a reading in
ppm Nitrite-Nitrogen then multiply the reading by 0.3 or use the table given below.
Higher Sensitivity
After your aquarium or garden pond has cycled you will need a much higher sensitivity. This is easily accomplished by looking through the side when comparing colors. Hole the test tube with its side against a white piece of color chart. The readings have to be divided by 10. In this way you will be able to detect 0.01
ppm as
nitrite or 0.003
ppm as
nitrite-nitrogen easily.
Nitrite Table
ppm Nitrite ppm Nitrite-Nitrogen
0.1 (too high) 0.03
0.2 0.06
0.4 (danger) 0.12
1.0 0.30
2.0 0.60
4.0 1.20
Iodine Test (measures iodide, iodate and iodine)
(1) Add with the syringe 4 ml of aquarium water in the small empty flask.
(2) Add to this 5 drops of I2-1 and swirl the contents of the flask for a few seconds.
(3) Add to this I2-2 dropwise. Swirl gently for a few seconds after each drop. Stop adding I2-2 when the water in the flask has a (faint) pink color. It does not matter how faint the color is. Look from above the test flask keeping a piece of white paper beneath it. Many aquarium waters will require one or two drops of I2-2. Allow to stand for 30 seconds.
(4) Add to this I2-3 drop wise. Swirl the flask gently for a few seconds after each drop. Continue adding I2-3 until the pink color has vanished completely. Add 2 more drops of I2-3 after the pink color has vanished. Swirl the flask gently for a few seconds. Cap the flask and shake the flask for 30 - 40 seconds. Proceed then within 30 seconds with the next step.
(5) Add to this 5 drops of I2-4 and with the plastic pipette 0.5 ml (= mark halfway the stem) of I2-5. Close the flasks immediately.
(6) Shake the test flask very strongly for 30 seconds. Allow it to stand for one more minute. A small layer of liquid will appear on top of the water layer. The coloration of the uppermost liquid layer is a measure for the
iodine content.
(7) Hold the test flask in front of you and keep the cap closed. Look through the side of the vial and compare the color of the upper liquid layer with that of the color chart. Hold a white piece (in between the 3 or 4 colors) of the color chart approx. 1 inch behind the test vial. The values on the color chart are in
ppm iodide.
Note: Natural seawater contains approx. 0.06
ppm iodine as iodide and iodate. Salifert's
Iodine contains both of them in the correct ratio.
Warning: Reagent I2-5 is inflammable. Keep away from fire and store in a cool place.
Ammonia
Instructions:
1. Add with 5ml syringe 1 ml of water in the test vial.
2. Add 6 drops of
NH3-1 (watch out caustic!) and swirl gently for 5 seconds.
3. Add 6 drops of
NH3-2 (watch out contains Mercury salts, wash hands after use, keep away from food, see additional warning under the heading WARNING). Swirl gently for 5 seconds and allow to stand for 5 minutes.
4. Hold the test vital in front of you and look through the side of the test vial. Keep the color chart behind the test vial. Ensure that a white piece of the color chart is behind the liquid layer. The values on the color chart are in mg/L total
ammonia (that is
NH3 + NH4+).
Magnesium
1. Add with the 5 ml syringe 3 ml of water in the test vial.
2. Add 6 drops of Mg-1 and swirl gently for 30 seconds.
3. Add 1 spoon of Mg-2 powder (spoon inside) to test vial and swirl for 10 seconds.
4. Place the plastic tip firmly on the 1 ml syringe and draw into this Mg-3 reagent until the lower end of the black syringe part is at the 1.00 ml mark. Ensure that during this that the plastic tip is submersed in the Mg-3 reagent to avoid that air bubbles are withdrawn instead of liquid. An air layer between the liquid and the piston is normal.
This is air which was present between the end of the tip and the piston, this will not influence the result.
5. Start adding the Mg-3 reagent with the 1 ml syringe to the testvial until the color changes to gray or blue (whichever color is observed first). Do this drop wise and swirl after each drop for a second or two.
6. Hold the syringe with the tip facing upward and read the position of the upper end of the black scringe part. Each division corresponds to 0.01 ml. The magnesium concentration can be obtained from the table or by use of the following equation:
ppm Mg = (1 - reading in step 6) x 1500
Natural sea water has a magnesium concentration of approx. 1300 - 1500
ppm. The concentration varies with
salinity.
Too low magnesium concentration makes maintaining correct calcium and alkalinity concentration difficult. Magnesium concentration can be increased with Salifert's magnesium.
Magnesium Table
Note: If you took 1.5 ml of water in step 1 then multiply the calcium values by 2!
Reading in ml's (step 6) Magnesium Concentration in
ppm
0.00............................. 1500
0.02............................. 1470
0.04 .............................1440
0.06............................. 1410
0.08 .............................1380
0.10 .............................1350
0.12 .............................1320
0.14 .............................1290
0.16 .............................1260
0.18 .............................1230
0.20............................. 1200
0.22............................. 1170
0.24 .............................1140
0.26 .............................1110
0.28 .............................1080
0.30 .............................1050
0.32 .............................1020
0.34 ............................. 990
0.36 ............................. 960
0.38.............................. 930
0.40 ............................. 900
0.42 ............................. 870
0.44 ............................. 840
0.46 ............................. 810
0.48 ............................. 780
0.50 ............................. 750
0.52 ............................. 720
0.54.............................. 690
0.56 ............................. 660
0.58 ............................. 630
0.60 ............................. 600
0.62 ............................. 570
0.64 ............................. 540
0.66.............................. 510
0.68 ............................. 480
0.70 ............................. 450
0.72 ............................. 420
0.74.............................. 390
0.76 ............................. 360
0.78.............................. 330
0.80 ............................. 300
0.82 ............................. 270
0.84 ............................. 240
0.86 ............................. 210
0.88 ............................. 180
0.90 ............................. 150
0.92.............................. 120
0.94 ............................. 90
0.96 ............................. 60
0.98.............................. 30