Does anyone have any pictures or know how to tell when you should split your T. Iso cultures? Mine gets an almost golden yellow color but doesnt seem to get much darker. I have tryed to add more F/2 media and it doesnt seem to help. Any suggestions? It isnt falling out of suspension or anything like that just doesnt seem to get a dark brown like Im expecting. How long should a healthy culture take to turn from light yellow to dark brown?

my understanding is it should be a nice golden brown, but my cultures are still new and im working up to that density, so i cant provide much in the form of experience or pictures, sorry..

i did ask Erik to jump in here though

http://i74.photobucket.com/albums/i273/bmwaaron/Tisoweb.jpg

This is my culture after 4 days.... Is it on track?

to my eye (still new to this strain)
id say it looks good to me.. :D

Looks like oragne koolaid

MMmmmm lets get the dixie cups and sit on the porch.. :)

I think the culture looks good especially after 4 days. I would typically renutrify after 5-6 days. The only other thought I have would be the amount of light it is getting...but judging from the picture not sure that is an issue. What kind of bulbs are you using? What are temps like? Our cultures darken to brown at 1 week-ish.

The temp stays around 76-80 F. I only have the lights on about 16 hours a day but am thinking about bumping that up. The reason I am a little worried is I did a culture about 2 weeks ago that didnt seem to get any darker then what is in the picture.