| Once you get your egg bundles from either species, you will have to give them 10-20 minutes to break up into eggs and sperm, probably less time by the time you get back to shore. I know that palmata will not self fertilize so you will have to get egg bundles from at least two separate colonies and make sure that they are far enough apart that there is a low likelihood that they are clones. Once your egg bundles break up and you have thoroughly mixed them all up in the same container (per species), give them about at least 1-2 hours to simmer with occasional gentle stirring. After that time, you will need to gently rinse off the still buyoant newly fertilized eggs several times to wash out the sperm. Before rinsing the egg-sperm water will be cloudy but when the rinses have washed off most of the sperm the water will become clear. If you do not rinse the egg-sperm water there is a risk that some eggs will be fertilized multiple times (polyspermy) which will result in unviable dead eggs. |