| Most people consider Nannochloropsis to be a weed. With that in mind T. Iso and Tetraselmis (Tet) are not, but they are not really that difficult to culture. They all use the same media. Tet likes it a bit cooler(18-25C) than T. Iso(18-26/27C). They are both swimmers. With Tet one can leave the aeration off for the first day. It seems that when an inoc goes into fresh media the cells drop out of suspension...but still divide. After day1 -2 turn on the air and the culture should be set to go. With T. Iso, we drop the cultures back about 4-5 inches from the light and then bring them up to the light after day1 or 2 depending on how dense the inoc was. I feel that both these microalgae are worth the effort.
Since these guys are swimmers, healthy cells will be in the water column and so performing a transfer could be as simple as pouring off the top of a select culture into the next culture vessel. | 
